 Chris PrattMario (voice) | Mario (voice) |
 Charlie DayLuigi (voice) | Luigi (voice) |
 Anya Taylor-JoyPrincess Peach (voice) | Princess Peach (voice) |
 Jack BlackBowser (voice) | Bowser (voice) |
 Keegan-Michael KeyToad (voice) | Toad (voice) |
 Donald GloverYoshi (voice) | Yoshi (voice) |
 Benny SafdieBowser Junior (voice) | Bowser Junior (voice) |
 Brie LarsonRosalina (voice) | Rosalina (voice) |
 Glen PowellFox McCloud (voice) | Fox McCloud (voice) |
 Luis GuzmánWart (voice) | Wart (voice) |
 Issa RaeHoney Queen (voice) | Honey Queen (voice) |
 Kevin Michael RichardsonKamek (voice) | Kamek (voice) |
| JJuliet JelenicLumalee (voice) | Lumalee (voice) |
| EEd SkudderR.O.B. (voice) | R.O.B. (voice) |
 Roxana OrtegaUkiki (voice) | Ukiki (voice) |
| VVirginia Dare JelenicYellow Luma (voice) | Yellow Luma (voice) |
 Eric BauzaToad General / Pajama Toad (voice) | Toad General / Pajama Toad (voice) |
 Carlos AlazraquiAdditional Voices (voice) | Additional Voices (voice) |
 Hope LevyAdditional Voices (voice) | Additional Voices (voice) |
 Monia AyachiAdditional Voices (voice) | Additional Voices (voice) |
| AAshley LondonAdditional Voices (voice) | Additional Voices (voice) |
 Ryan BartleyAdditional Voices (voice) | Additional Voices (voice) |
 Eric LopezAdditional Voices (voice) | Additional Voices (voice) |
 Rachel ButeraAdditional Voices (voice) | Additional Voices (voice) |
 Scott MenvilleAdditional Voices (voice) | Additional Voices (voice) |
 Jessica DiCiccoAdditional Voices (voice) | Additional Voices (voice) |
 Laraine NewmanAdditional Voices (voice) | Additional Voices (voice) |
 Johnny GidcombAdditional Voices (voice) | Additional Voices (voice) |
 Matt NolanAdditional Voices (voice) | Additional Voices (voice) |
 Danielle HartnettAdditional Voices (voice) | Additional Voices (voice) |
