 Chris PrattMario (voice) | Mario (voice) |
 Anya Taylor-JoyPrincess Peach (voice) | Princess Peach (voice) |
 Charlie DayLuigi (voice) | Luigi (voice) |
 Jack BlackBowser (voice) | Bowser (voice) |
 Keegan-Michael KeyToad (voice) | Toad (voice) |
 Seth RogenDonkey Kong (voice) | Donkey Kong (voice) |
 Fred ArmisenCranky Kong (voice) | Cranky Kong (voice) |
 Sebastian ManiscalcoSpike (voice) | Spike (voice) |
 Charles MartinetMario's Dad / Giuseppe (voice) | Mario's Dad / Giuseppe (voice) |
 Kevin Michael RichardsonKamek (voice) | Kamek (voice) |
 Khary PaytonPenguin King (voice) | Penguin King (voice) |
 Rino RomanoUncle Tony (voice) | Uncle Tony (voice) |
 John DiMaggioUncle Arthur (voice) | Uncle Arthur (voice) |
 Jessica DiCiccoMario's Mom (voice) | Mario's Mom (voice) |
 Eric BauzaToad General (voice) | Toad General (voice) |
| JJuliet JelenicLumalee (voice) | Lumalee (voice) |
 Scott MenvilleKoopa General (voice) | Koopa General (voice) |
 Carlos AlazraquiAdditional Voices (voice) | Additional Voices (voice) |
| JJason BroadAdditional Voices (voice) | Additional Voices (voice) |
 Ashly BurchAdditional Voices (voice) | Additional Voices (voice) |
 Rachel ButeraAdditional Voices (voice) | Additional Voices (voice) |
 Cathy CavadiniAdditional Voices (voice) | Additional Voices (voice) |
 Will CollyerAdditional Voices (voice) | Additional Voices (voice) |
| DDjango CraigAdditional Voices (voice) | Additional Voices (voice) |
 Willow GeerAdditional Voices (voice) | Additional Voices (voice) |
 Aaron HendryAdditional Voices (voice) | Additional Voices (voice) |
 Andy HirschAdditional Voices (voice) | Additional Voices (voice) |
 Barbara HarrisAdditional Voices (voice) | Additional Voices (voice) |
 Phil LaMarrAdditional Voices (voice) | Additional Voices (voice) |
 Jeremy MaxwellAdditional Voices (voice) | Additional Voices (voice) |
