 Tobey MaguireSpider-Man / Peter Parker | Spider-Man / Peter Parker |
 Kirsten DunstMary Jane Watson | Mary Jane Watson |
 James FrancoNew Goblin / Harry Osborn | New Goblin / Harry Osborn |
 Thomas Haden ChurchSandman / Flint Marko | Sandman / Flint Marko |
 Topher GraceVenom / Eddie Brock | Venom / Eddie Brock |
 Bryce Dallas HowardGwen Stacy | Gwen Stacy |
 Rosemary HarrisMay Parker | May Parker |
 J.K. SimmonsJ. Jonah Jameson | J. Jonah Jameson |
 James CromwellCaptain Stacy | Captain Stacy |
 Dylan BakerDr. Curt Connors | Dr. Curt Connors |
 Theresa RussellEmma Marko | Emma Marko |
 Bill NunnJoseph 'Robbie' Robertson | Joseph 'Robbie' Robertson |
 Bruce CampbellMaître D' | Maître D' |
 Elizabeth BanksMiss Brant | Miss Brant |
 Ted RaimiHoffman | Hoffman |
 Perla Haney-JardinePenny Marko | Penny Marko |
 Willem DafoeGreen Goblin / Norman Osborn | Green Goblin / Norman Osborn |
 Cliff RobertsonBen Parker | Ben Parker |
 Elya BaskinMr. Ditkovitch | Mr. Ditkovitch |
 Mageina TovahUrsula | Ursula |
 John PaxtonHouseman | Houseman |
 Becky Ann BakerMrs. Stacy | Mrs. Stacy |
 Stan LeeMan in Times Square | Man in Times Square |
 Michael PapajohnDennis Carradine / Carjacker | Dennis Carradine / Carjacker |
 Joe ManganielloFlash Thompson | Flash Thompson |
| HHal FishmanAnchorman | Anchorman |
 Lucy GordonJennifer Dugan | Jennifer Dugan |
 Steve ValentinePhotographer | Photographer |
 Tim MaculanPlay Director | Play Director |
 Marc VannPlay Producer | Play Producer |
