The second night of New Japan Cup 2021 took place on March 6, 2021, at Ota City General Gymnasium in Tokyo, Japan, and featured three first-round matches.
| Actor | Role |
|---|---|
 Shin TakagiShingo Takagi | Shingo Takagi |
 Kazuchika OkadaKazuchika Okada | Kazuchika Okada |
 Kenta KobayashiKENTA | KENTA |
 Joseph RobinsonJuice Robinson | Juice Robinson |
 Tomoaki HonmaTomoaki Honma | Tomoaki Honma |
 Minoru SuzukiMinoru Suzuki | Minoru Suzuki |
 Tetsuya ShimizuBUSHI | BUSHI |
 Seiya SanadaSANADA | SANADA |
 Tetsuya NaitoTetsuya Naito | Tetsuya Naito |
 Tomoyuki OkaGreat-O-Khan | Great-O-Khan |
 Jeffrey CobbJeff Cobb | Jeff Cobb |
 William OspreayWill Ospreay | Will Ospreay |
 David Stephen Finlay IIIDavid Finlay | David Finlay |
 Aaron HenryToa Henare | Toa Henare |
 Yuya UemuraYuya Uemura | Yuya Uemura |
 Steven OwensChase Owens | Chase Owens |
 Jamie WhiteJay White | Jay White |
 Taiji IshimoriTaiji Ishimori | Taiji Ishimori |