 Tara StrongSci-Twi / Midnight Sparkle / Twilight Sparkle (voice) | Sci-Twi / Midnight Sparkle / Twilight Sparkle (voice) |
 Rebecca ShoichetSunset Shimmer (voice) / Sci-Twi (singing voice) | Sunset Shimmer (voice) / Sci-Twi (singing voice) |
 Ashleigh BallApplejack / Rainbow Dash (voice) | Applejack / Rainbow Dash (voice) |
 Andrea LibmanPinkie Pie / Fluttershy / Bon Bon (voice) | Pinkie Pie / Fluttershy / Bon Bon (voice) |
 Tabitha St. GermainRarity / Vice Principal Luna (voice) | Rarity / Vice Principal Luna (voice) |
 Cathy WeseluckSpike (voice) | Spike (voice) |
 Iris QuinnPrincipal Abacus Cinch (voice) | Principal Abacus Cinch (voice) |
 Nicole OliverPrincipal Celestia (voice) | Principal Celestia (voice) |
 Britt McKillipDean Cadance (voice) | Dean Cadance (voice) |
 Vincent TongFlash Sentry / Bus Driver (voice) | Flash Sentry / Bus Driver (voice) |
 Andrew FrancisShining Armor (voice) | Shining Armor (voice) |
 Sienna BohnSugarcoat (voice) | Sugarcoat (voice) |
| SSharon AlexanderSour Sweet (voice) | Sour Sweet (voice) |
 Kelly SheridanIndigo Zap (voice) | Indigo Zap (voice) |
 Britt IrvinSunny Flare (voice) | Sunny Flare (voice) |
 Shannon Chan-KentLemon Zest (voice) / Pinkie Pie (singing voice) | Lemon Zest (voice) / Pinkie Pie (singing voice) |
 Kazumi EvansRarity (singing voice) | Rarity (singing voice) |
 Lili BeaudoinTennis Match (voice) | Tennis Match (voice) |
 Diana KaarinaMystery Mint (voice) | Mystery Mint (voice) |
| IIngrid NilsonSweet Leaves (voice) | Sweet Leaves (voice) |
 Kaylee JohnstonBackground singer (voice uncredited) | Background singer (voice uncredited) |
