 Greg EaglesGrim / Sperg (voice) | Grim / Sperg (voice) |
 Richard Steven HorvitzBilly / Billy's Dad (voice) | Billy / Billy's Dad (voice) |
 Grey DeLisleMandy (voice) | Mandy (voice) |
 Vanessa MarshallIrwin (voice) | Irwin (voice) |
 Kari WahlgrenVelma Green the Spider Queen (voice) | Velma Green the Spider Queen (voice) |
 Maxwell AtomsJeff the Spider (voice) | Jeff the Spider (voice) |
 Dee Bradley BakerChocolate Cow (voice) | Chocolate Cow (voice) |
 Jennifer HaleGladysaur (voice) | Gladysaur (voice) |
 Chris CoxPrincipal Goodvibes (voice) | Principal Goodvibes (voice) |
 Jane CarrPud'n (voice) | Pud'n (voice) |
 Henry GibsonLord Pain (voice) | Lord Pain (voice) |
 Phil LaMarrMr. Spleen (voice) | Mr. Spleen (voice) |
 Rachael MacFarlaneMindy / Velma's Mom / Eris (voice) | Mindy / Velma's Mom / Eris (voice) |
 Michael McKeanKalgoron (voice) | Kalgoron (voice) |
| RRenee RaudmanMs. Butterbean (voice) | Ms. Butterbean (voice) |
 David L. LanderCurtis (voice) | Curtis (voice) |
 Fred WillardBoogey (voice) | Boogey (voice) |
 James SiePlain Cow (voice) | Plain Cow (voice) |
 Armin ShimermanGeneral Skarr (voice) | General Skarr (voice) |
 Dave WittenbergVelma's Dad / Spider #1 (voice) | Velma's Dad / Spider #1 (voice) |
