After the conclusion of the Battle City Tournament, deep below the sands of Egypt, an ancient evil has awakened. Anubis, who was defeated centuries ago by Yugi’s mysterious alter ego – the ancient Pharaoh – has returned for revenge. Wielding the power of the Eighth Millennium Item, Anubis is determined to destroy Yugi and take over the world.
| Actor | Role |
|---|---|
 Dan GreenYugi Mutou / Yami Yugi (voice) | Yugi Mutou / Yami Yugi (voice) |
 Eric StuartSeto Kaiba (voice) | Seto Kaiba (voice) |
| SScott RayowAnubis (voice) | Anubis (voice) |
 Wayne GraysonJoey Wheeler (voice) | Joey Wheeler (voice) |
 Gregory AbbeyTristan Taylor (voice) | Tristan Taylor (voice) |
 Amy BirnbaumTéa Gardner (voice) | Téa Gardner (voice) |
 Tara SandsMokuba Kaiba (voice) | Mokuba Kaiba (voice) |
 Maddie BlausteinSolomon Muto (voice) | Solomon Muto (voice) |
 Darren DunstanMaximillion Pegasus (voice) | Maximillion Pegasus (voice) |
 Christopher ColletAdditional Voices | Additional Voices |
 Sebastian ArcelusAdditional Voices | Additional Voices |
 Lisa OrtizAdditional Voices | Additional Voices |
 Ted LewisAdditional Voices | Additional Voices |
 Scott RayowAnubis (voice) | Anubis (voice) |