During an ordinary day in Hundred Acre Wood, Winnie the Pooh sets out to find some honey. Misinterpreting a note from Christopher Robin, Owl convinces Pooh, Tigger, Rabbit, Piglet, Kanga, Roo, and Eeyore that their young friend has been captured by a creature named "Backson" and they set out to rescue him.
| Actor | Role |
|---|---|
 Jim CummingsWinnie the Pooh / Tigger (voice) | Winnie the Pooh / Tigger (voice) |
 Bud LuckeyEeyore (voice) | Eeyore (voice) |
 Craig FergusonOwl (voice) | Owl (voice) |
 Travis OatesPiglet (voice) | Piglet (voice) |
 Tom KennyRabbit (voice) | Rabbit (voice) |
 Kristen Anderson-LopezKanga (voice) | Kanga (voice) |
| WWyatt Dean HallRoo (voice) | Roo (voice) |
 Jack BoulterChristopher Robin (voice) | Christopher Robin (voice) |
 Huell HowserBackson (voice) | Backson (voice) |
 John CleeseNarrator (voice) | Narrator (voice) |
| LLisa LinderAdditional Voices (voice) | Additional Voices (voice) |
 Robert LopezAdditional Voices (voice) | Additional Voices (voice) |
 Zooey Deschaneladditional voice | additional voice |
 M. Wardadditional voice | additional voice |