Viridiana is preparing to start her life as a nun when she is sent, somewhat unwillingly, to visit her aging uncle, Don Jaime. He supports her; but the two have met only once. Jaime thinks Viridiana resembles his dead wife. Viridiana has secretly despised this man all her life and finds her worst fears proven when Jaime grows determined to seduce his pure niece. Viridiana becomes undone as her uncle upends the plans she had made to join the convent.
| Actor | Role |
|---|---|
 Silvia PinalViridiana | Viridiana |
 Francisco RabalJorge | Jorge |
 Fernando ReyDon Jaime | Don Jaime |
 José CalvoDon Amalio | Don Amalio |
 Margarita LozanoRamona | Ramona |
 Victoria ZinnyLucía | Lucía |
 Teresa RabalRita | Rita |
| LLuis HerediaEl Poca | El Poca |
 Joaquín RoaDon Zequiel | Don Zequiel |
 José Manuel MartínEl Cojo | El Cojo |
 Lola GaosEnedina | Enedina |
| JJuan García TiendraJosé 'El Leproso' (uncredited) | José 'El Leproso' (uncredited) |
 Sergio MendizábalEl Pelón | El Pelón |
 María IsbertBeggar | Beggar |
 Claudio Brook(uncredited) | (uncredited) |
| NNarciso Ojeda(uncredited) | (uncredited) |
 José María LadoMayor (uncredited) | Mayor (uncredited) |
 Rosita YarzaMother Superior (uncredited) | Mother Superior (uncredited) |