Rancher Rex Allen receives a summons from his uncle. an old time frontiersman, that he is in trouble. The uncle has been hired to lead a modern-day band of adventurers on a wagon train retracing the route taken by their ancestors 100 years ago. Before Rex can talk to his uncle, the uncle is murdered, and Rex sets out to find the killer and the motive by taking his uncle's place as the leader of the wagon train.
| Actor | Role |
|---|---|
 Rex AllenRex Allen | Rex Allen |
 KokoKoko, Rex Allen's Horse (as Koko the Miracle Horse of the Movies) | Koko, Rex Allen's Horse (as Koko the Miracle Horse of the Movies) |
 Buddy Ebsen'Snooper' Trent | 'Snooper' Trent |
 Penny EdwardsNancy Bonner | Nancy Bonner |
 Roy BarcroftHank Driscoll | Hank Driscoll |
 Sarah PaddenSarah Wendover | Sarah Wendover |
 Grant WithersRed Bancroft | Red Bancroft |
 Arthur SpaceRobert Hatfield | Robert Hatfield |
| EEdwin RandJess Sutton | Jess Sutton |
 Robert KarnesHenchman Jack Scully | Henchman Jack Scully |
| WWilliam HolmesHenchman Pete Millan | Henchman Pete Millan |
 Stanley AndrewsSheriff | Sheriff |
 Frank JenksHap - Telephone Company Lineman | Hap - Telephone Company Lineman |
 Al BridgeSam Sickle | Sam Sickle |
 Forrest TaylorCyrus Bonner | Cyrus Bonner |