The Two Senoritas from Chicago are Gloria and Maria. When their goofy pal Daisy Baker passes off a discarded Portuguese play manuscript as her own, producer Rupert Shannon agrees to bankroll the production. With stars in their eyes, Gloria and Maria pretend to be a pair of Portuguese musical comedy stars, thereby winning parts in the new production. The fun begins when the play's original authors sell the same manuscript to a rival producer.
| Actor | Role |
|---|---|
 Joan DavisDaisy Baker | Daisy Baker |
 Jinx FalkenburgGloria | Gloria |
 Ann SavageMaria | Maria |
 Leslie BrooksLena Worth | Lena Worth |
 Ramsay AmesLouise Hotchkiss | Louise Hotchkiss |
 Bob HaymesJeff Kenyon | Jeff Kenyon |
 Emory ParnellRupert Shannon | Rupert Shannon |
 Douglas LeavittSam Grohman | Sam Grohman |
| MMuni SeroffGilberto Garcia | Gilberto Garcia |