 Michael SinterniklaasLeonardo (voice) | Leonardo (voice) |
 Wayne GraysonMichelangelo (voice) | Michelangelo (voice) |
 Sam RiegelDonatello (voice) | Donatello (voice) |
 Gregory AbbeyRaphael (voice) | Raphael (voice) |
 Darren DunstanSplinter (voice) | Splinter (voice) |
 Marc ThompsonCasey Jones (voice) | Casey Jones (voice) |
 Veronica TaylorApril O'Neil (voice) | April O'Neil (voice) |
 Scott RayowThe Shredder (voice) | The Shredder (voice) |
| GGreg CareyHun (voice) | Hun (voice) |
| KKaren NeilKarai (voice) | Karai (voice) |
 Dan Green1988 Leonardo (voice) | 1988 Leonardo (voice) |
| JJohnny Castro1988 Michelangelo / Rocksteady (voice) | 1988 Michelangelo / Rocksteady (voice) |
| AAnthony Salerno1988 Donatello (voice) | 1988 Donatello (voice) |
 Sebastian Arcelus1988 Raphael (voice) | 1988 Raphael (voice) |
 David Wills1988 Splinter / 1984 Shredder (voice) | 1988 Splinter / 1984 Shredder (voice) |
 Rebecca Soler1988 April O'Neil (voice) | 1988 April O'Neil (voice) |
| LLoad Williams1988 Shredder (voice) | 1988 Shredder (voice) |
| BBradford ScobieKrang / Bebop / 1984 Michelangelo (voice) | Krang / Bebop / 1984 Michelangelo (voice) |
 Jason Griffith1984 Leonardo / Additional Voices (voice) | 1984 Leonardo / Additional Voices (voice) |
 Sean Schemmel1984 Raphael / Additional Voices (voice) | 1984 Raphael / Additional Voices (voice) |
| CClay Adams1984 Donatello (voice) | 1984 Donatello (voice) |
| BBruce FalkAdditional Voices (voice) | Additional Voices (voice) |
| MMatthew PiazziAdditional Voices (voice) | Additional Voices (voice) |
 Kevin EastmanKevin Eastman (voice) (uncredited) | Kevin Eastman (voice) (uncredited) |
 Peter LairdPeter Laird (voice) (uncredited) | Peter Laird (voice) (uncredited) |
