 Nova PilbeamLady Jane Grey | Lady Jane Grey |
 Cedric HardwickeEarl of Warwick | Earl of Warwick |
 John MillsLord Guilford Dudley | Lord Guilford Dudley |
 Felix AylmerEdward Seymour | Edward Seymour |
 Leslie PerrinsThomas Seymour | Thomas Seymour |
 Frank CellierHenry VIII | Henry VIII |
 Desmond TesterEdward VI | Edward VI |
 Gwen Ffrangcon DaviesMary Tudor (as Gwen Ffrangcon-Davies) | Mary Tudor (as Gwen Ffrangcon-Davies) |
 Martita HuntJane's Mother | Jane's Mother |
| MMiles MallesonJane's Father | Jane's Father |
 Sybil ThorndikeEllen | Ellen |
| PPeter CroftConfidant of Thomas Seymour | Confidant of Thomas Seymour |
| AAlbert DaviesBarnaby Fitzpatrick | Barnaby Fitzpatrick |
| SShaun DesmondUndetermined Role | Undetermined Role |
| EEdward DignonUndetermined Role | Undetermined Role |
 Roy EmertonSquire | Squire |
 C. V. FranceClergy at Execution | Clergy at Execution |
| AArthur GoulletSir John Gates | Sir John Gates |
| HHenry HallettUndetermined Role | Undetermined Role |
 John LaurieJohn Knox | John Knox |
| EEugene LeahyUndetermined Role | Undetermined Role |
| FFewlass LlewellynUndetermined Role | Undetermined Role |
| CCicely Paget-BowmanCourtier | Courtier |
 John SingerBoy | Boy |
 John TurnbullArundel | Arundel |
