 Frank WelkerScooby-Doo / Rudy / Fred / Nefario (voice) | Scooby-Doo / Rudy / Fred / Nefario (voice) |
 Grey DeLisleDaphne / Daisy / Musketeer 1 / Olive (voice) | Daphne / Daisy / Musketeer 1 / Olive (voice) |
 Matthew LillardShaggy / Craggly / Captain Cutler (voice) | Shaggy / Craggly / Captain Cutler (voice) |
 Kate MicucciVelma / Helga (voice) | Velma / Helga (voice) |
 Myrna VelascoCoco Diablo (voice) | Coco Diablo (voice) |
 Dee Bradley BakerEsteban / Mr. Wickles / Cat Man (voice) | Esteban / Mr. Wickles / Cat Man (voice) |
 Jeff BennettShady Guy / Charlie Humdrum / Hank (voice) | Shady Guy / Charlie Humdrum / Hank (voice) |
 Anthony CarriganTrevor Glume / Farmer (voice) | Trevor Glume / Farmer (voice) |
 Erin FitzgeraldLibrary Kid / Superhero Girl / Musketeer 2 (voice) | Library Kid / Superhero Girl / Musketeer 2 (voice) |
 David LodgeWarden Collins / Harry the Hypnotist / Inspector (voice) | Warden Collins / Harry the Hypnotist / Inspector (voice) |
 Lara Jill MillerPrisoner Costume Teen / Superhero Boy / Musketeer 3 (voice) | Prisoner Costume Teen / Superhero Boy / Musketeer 3 (voice) |
 Candi MiloPrison Woman / Dinosaur Kid / Monster Kid (voice) | Prison Woman / Dinosaur Kid / Monster Kid (voice) |
| JJenelle Lynn RandallLibrarian / Superboy (voice) | Librarian / Superboy (voice) |
 Kevin Michael RichardsonSheriff / Henry Bascombe / 10,000 Volt Ghost (voice) | Sheriff / Henry Bascombe / 10,000 Volt Ghost (voice) |
