Autobots, Decepticons, Predaking and Predacons form an unlikely alliance to battle a resurrected Unicron.
| Actor | Role |
|---|---|
 Peter CullenOptimus Prime (voice) | Optimus Prime (voice) |
 Frank WelkerMegatron / Terrorcon (voice) | Megatron / Terrorcon (voice) |
 Steve BlumStarscream / Darksteel (voice) | Starscream / Darksteel (voice) |
 Jeffrey CombsRatchet (voice) | Ratchet (voice) |
 Will FriedleBumblebee (voice) | Bumblebee (voice) |
 James HoranWheeljack (voice) | Wheeljack (voice) |
 Michael IronsideUltra Magnus (voice) | Ultra Magnus (voice) |
 Peter MensahPredaking (voice) | Predaking (voice) |
 Sumalee MontanoArcee (voice) | Arcee (voice) |
 John NobleUnicron (voice) | Unicron (voice) |
 Daran NorrisKnock Out (voice) | Knock Out (voice) |
 Nolan NorthSmokescreen / Skylynx (voice) | Smokescreen / Skylynx (voice) |
 Kevin Michael RichardsonBulkhead (voice) | Bulkhead (voice) |
 David SobolovShockwave (voice) | Shockwave (voice) |