 Chris EvansCasey Jones (voice) | Casey Jones (voice) |
 Sarah Michelle GellarApril O'Neil (voice) | April O'Neil (voice) |
 Patrick StewartWinters (voice) | Winters (voice) |
 MakoSplinter (voice) | Splinter (voice) |
 Zhang ZiyiKarai (voice) | Karai (voice) |
 James Arnold TaylorLeonardo (voice) | Leonardo (voice) |
 Mitchell WhitfieldDonatello / Additional Voices (voice) | Donatello / Additional Voices (voice) |
 Nolan NorthRaphael / Nightwatcher (voice) | Raphael / Nightwatcher (voice) |
 Mikey KelleyMichelangelo (voice) | Michelangelo (voice) |
 Laurence FishburneNarrator (voice) | Narrator (voice) |
 Kevin SmithDiner Cook (voice) | Diner Cook (voice) |
 John DiMaggioColonel Santino (voice) | Colonel Santino (voice) |
| PPaula MattioliGeneral Serpiente (voice) | General Serpiente (voice) |
 Kevin Michael RichardsonGeneral Aguila / Additional Voices (voice) | General Aguila / Additional Voices (voice) |
 Fred TatascioreGeneral Gato / Additional Voices (voice) | General Gato / Additional Voices (voice) |
 Dee Bradley BakerAdditional Voices (voice) | Additional Voices (voice) |
 Greg BaldwinSplinter (voice) | Splinter (voice) |
 Jeff BennettAdditional Voices (voice) | Additional Voices (voice) |
 Jim CummingsAdditional Voices (voice) | Additional Voices (voice) |
 Grey DeLisleAdditional Voices (voice) | Additional Voices (voice) |
 Chris EdgerlyAdditional Voices (voice) | Additional Voices (voice) |
 Kim Mai GuestAdditional Voices (voice) | Additional Voices (voice) |
 Jennifer HaleAdditional Voices (voice) | Additional Voices (voice) |
 Jess HarnellAdditional Voices (voice) | Additional Voices (voice) |
 Phil LaMarrAdditional Voices (voice) | Additional Voices (voice) |
 Paul Michael RobinsonAdditional Voices (voice) | Additional Voices (voice) |
 Tara StrongAdditional Voices (voice) | Additional Voices (voice) |
 Billy WestAdditional Voices (voice) | Additional Voices (voice) |
 Kevin MunroeDiner Patron (voice) (uncredited) | Diner Patron (voice) (uncredited) |
