| JJohn HaslerThomas (voice) | Thomas (voice) |
 Keith WickhamEdward / Henry / Gordon / Bertie / Harold / Captain / The Fat Controller / Dowager Hatt / The Thin Controller (voice) | Edward / Henry / Gordon / Bertie / Harold / Captain / The Fat Controller / Dowager Hatt / The Thin Controller (voice) |
 Rob RackstrawJames / Toby / Donald / Sam / Big Mickey / The Thin Clergyman / African Race Announcer (voice) | James / Toby / Donald / Sam / Big Mickey / The Thin Clergyman / African Race Announcer (voice) |
 Nigel PilkingtonPercy (voice) | Percy (voice) |
 Steven KynmanDuck / Paxton (voice) | Duck / Paxton (voice) |
 Teresa GallagherEmily / Marion / Daisy / Natalie / American Diesel / Annie / Clarabel / Cassia (voice) | Emily / Marion / Daisy / Natalie / American Diesel / Annie / Clarabel / Cassia (voice) |
 Tim WhitnallBill / Timothy / Moai (voice) | Bill / Timothy / Moai (voice) |
| MMatt WilkinsonBen / Cranky / Tony / Carter (voice) | Ben / Cranky / Tony / Carter (voice) |
 Rufus JonesThe Flying Scotsman (voice) | The Flying Scotsman (voice) |
 Nicola StapletonRosie (voice) | Rosie (voice) |
 Dan LiYong Bao (voice) | Yong Bao (voice) |
| EEliud Gabriel PorrasCarlos / Fernando / Emerson / Brazilian Troublesome Trucks / Various (voice) | Carlos / Fernando / Emerson / Brazilian Troublesome Trucks / Various (voice) |
| YYvonne GrundyNia (voice) | Nia (voice) |
| PPatricia KihoroNia (singing voice) | Nia (singing voice) |
 Kerry ShaleBeau / Diesel (voice) | Beau / Diesel (voice) |
| BBob GoldingSidney (voice) | Sidney (voice) |
 Rasmus HardikerBill / Philip (voice) | Bill / Philip (voice) |
| SSu Lin LooiChinese Diesel (voice) | Chinese Diesel (voice) |
 Lucy MontgomeryCarly / Cowgirls (voice) | Carly / Cowgirls (voice) |
 Rachael Louise MillerAngelique (voice) | Angelique (voice) |
 Abubakar SalimKwaku / African Van / Kobe / Various (voice) | Kwaku / African Van / Kobe / Various (voice) |
 Richie CampbellAfrican Van / Various (voice) | African Van / Various (voice) |
 Akiya HenryAfrican Tankers / Various (voice) | African Tankers / Various (voice) |
 Chipo ChungAfrican Van / Various (voice) | African Van / Various (voice) |
| LLinda AdweraVarious (voice) | Various (voice) |
 Peter AndreAce (voice) | Ace (voice) |
 David MenkinRacing Car / Cowboys (voice) | Racing Car / Cowboys (voice) |
| CChristopher RaglandRacing Car / Cowboys (voice) | Racing Car / Cowboys (voice) |
| JJoe MillsDouglas / Oliver (voice) (uncredited) | Douglas / Oliver (voice) (uncredited) |
