 Lacey ChabertEliza Thornberry (voice) | Eliza Thornberry (voice) |
 Tom KaneDarwin (voice) | Darwin (voice) |
 Cree SummerPhaedra (Elephant) (voice) | Phaedra (Elephant) (voice) |
 Tim CurryNigel Thornberry / Col. Radcliff Thornberry (voice) | Nigel Thornberry / Col. Radcliff Thornberry (voice) |
 Lynn RedgraveCordelia Thornberry (voice) | Cordelia Thornberry (voice) |
 Jodi CarlisleMarianne Thornberry (voice) | Marianne Thornberry (voice) |
 Danielle HarrisDebbie Thornberry (voice) | Debbie Thornberry (voice) |
 FleaDonnie Thornberry (voice) | Donnie Thornberry (voice) |
 Crystal ScalesCheetah Cubs (voice) | Cheetah Cubs (voice) |
 Kimberly BrooksTally (Cheetah Cub) (voice) | Tally (Cheetah Cub) (voice) |
 Alfre WoodardAkela (Cheetah Mother) (voice) | Akela (Cheetah Mother) (voice) |
 Brock PetersJomo (voice) | Jomo (voice) |
 Melissa GreenspanSarah Wellington (voice) | Sarah Wellington (voice) |
 Alexandra BoydVictoria (voice) | Victoria (voice) |
 Moira QuirkJane (voice) | Jane (voice) |
 Tara StrongSchoolgirl (voice) | Schoolgirl (voice) |
 Hynden WalchSchoolgirl (voice) | Schoolgirl (voice) |
 Mae WhitmanSchoolgirl (voice) | Schoolgirl (voice) |
 Roger L. JacksonReggie (Squirrel) / Thunder (voice) | Reggie (Squirrel) / Thunder (voice) |
 John KassirSquirrel (voice) | Squirrel (voice) |
 Charles ShaughnessySquirrel (voice) | Squirrel (voice) |
 Brenda BlethynMrs. Fairgood (voice) | Mrs. Fairgood (voice) |
 Kevin Michael RichardsonShaman Mnyambo (voice) | Shaman Mnyambo (voice) |
 Billy BrownRhino (voice) | Rhino (voice) |
 Marisa TomeiBree Blackburn (voice) | Bree Blackburn (voice) |
 Rupert EverettSloan Blackburn (voice) | Sloan Blackburn (voice) |
| JJeff CoopwoodTim (Park Ranger) (voice) | Tim (Park Ranger) (voice) |
| DDidier M. NgoleRanger / BaAka Villager (voice) | Ranger / BaAka Villager (voice) |
 Tony OkungbowaRanger (voice) | Ranger (voice) |
 James Brown OrleansZebu (voice) | Zebu (voice) |
