 Kay FrancisFlorence Nightingale | Florence Nightingale |
 Ian HunterFuller | Fuller |
 Donald WoodsCharles Cooper | Charles Cooper |
 Nigel BruceDr. West | Dr. West |
 Donald CrispDr. Hunt | Dr. Hunt |
 Henry O'NeillDr. Scott | Dr. Scott |
 Billy MauchTommy | Tommy |
 Charles Croker-KingMr. Nightingale | Mr. Nightingale |
 Phoebe FosterElizabeth Herbert | Elizabeth Herbert |
 George CurzonSidney Herbert | Sidney Herbert |
 Georgia CaineMrs. Nightingale | Mrs. Nightingale |
| AAra GeraldElla Stephens | Ella Stephens |
 Halliwell HobbesLord Raglan | Lord Raglan |
 Eily MalyonSister Colomba | Sister Colomba |
 Montagu LoveMr. Bullock | Mr. Bullock |
 Ferdinand MunierAlexis Soyer | Alexis Soyer |
 Lillian Kemble-CooperParthenope Nightingale | Parthenope Nightingale |
 Egon BrecherPastor Fliedner | Pastor Fliedner |
 Tempe PigottMrs. Waters | Mrs. Waters |
 Barbara LeonardMinna | Minna |
 Frank ConroyMr. Le Froy | Mr. Le Froy |
| LLowden AdamsSecretary (uncredited) | Secretary (uncredited) |
 Harry AllenSoldier Bothered By Rats (uncredited) | Soldier Bothered By Rats (uncredited) |
| DDorothy ArvilleNurse (uncredited) | Nurse (uncredited) |
 Jimmy AubreySentry (uncredited) | Sentry (uncredited) |
 Frank BakerCustoms Inspector (uncredited) | Customs Inspector (uncredited) |
 May BeattyNurse (uncredited) | Nurse (uncredited) |
 Daisy BelmoreNurse (uncredited) | Nurse (uncredited) |
 Lionel BelmoreCaptain (uncredited) | Captain (uncredited) |
| WWilson BengeJones (uncredited) | Jones (uncredited) |
