| JJim AmbroseSelf | Self |
| AAlice AmbroseSelf - Jim's Mother (archive footage) | Self - Jim's Mother (archive footage) |
| JJohn AmbroseSelf - Jim's Father (archive footage) | Self - Jim's Father (archive footage) |
| SSheilaSelf - Kristi's Soccer Coach | Self - Kristi's Soccer Coach |
| HHeatherSelf - Kristi's Childhood Friend | Self - Kristi's Childhood Friend |
| JJohn MoneySelf - Sexologist (archive footage | Self - Sexologist (archive footage |
| DDavid ReimerSelf (archive footage) | Self (archive footage) |
| BBrian ReimerSelf - David's Twin Brother (archive footage) (archive sound) | Self - David's Twin Brother (archive footage) (archive sound) |
| JJanet ReimerSelf - David's Mother (archive footage) | Self - David's Mother (archive footage) |
| RRichard G. CarterSelf - Retired Urologist | Self - Retired Urologist |
| TTiger DevoreSelf - Clinical Psychologist | Self - Clinical Psychologist |
| CCheryl ChaseSelf - Founder of the Intersex Society of North America | Self - Founder of the Intersex Society of North America |
| JJohn ColapintoSelf - Journalist | Self - Journalist |
| HH. Keith SigmundsonSelf - Head of Dept. of Psychiatry Child Guidance Clinic | Self - Head of Dept. of Psychiatry Child Guidance Clinic |
 Anderson CooperSelf - Host (archive footage) | Self - Host (archive footage) |
 Oprah WinfreySelf - Host (archive footage) | Self - Host (archive footage) |
