 Bette DavisQueen Elizabeth | Queen Elizabeth |
 Errol FlynnThe Earl of Essex | The Earl of Essex |
 Olivia de HavillandLady Penelope Gray | Lady Penelope Gray |
 Donald CrispFrancis Bacon | Francis Bacon |
 Alan HaleThe Prince of Tyrone | The Prince of Tyrone |
 Vincent PriceSir Walter Raleigh | Sir Walter Raleigh |
 Henry StephensonLord Burghley | Lord Burghley |
 Henry DaniellSir Robert Cecil | Sir Robert Cecil |
 James StephensonSir Thomas Egerton | Sir Thomas Egerton |
 Nanette FabrayMistress Margaret Radcliffe | Mistress Margaret Radcliffe |
 Ralph ForbesLord Knollys | Lord Knollys |
 Robert WarwickLord Mountjoy | Lord Mountjoy |
 Leo G. CarrollSir Edward Coke | Sir Edward Coke |
| GGuy BellisLord Charles Howard (uncredited) | Lord Charles Howard (uncredited) |
| JJacob DanceEssex's Soldier (uncredited) | Essex's Soldier (uncredited) |
 Forrester HarveyBit Part (uncredited) | Bit Part (uncredited) |
 Holmes HerbertMajordomo (uncredited) | Majordomo (uncredited) |
 I. Stanford JolleySpectator Outside Whitehall Palace (uncredited) | Spectator Outside Whitehall Palace (uncredited) |
 Doris LloydHandmaiden (uncredited) | Handmaiden (uncredited) |
 John SuttonCapt. Armand of the Queen's Guard (uncredited) | Capt. Armand of the Queen's Guard (uncredited) |
 Rosella TowneLady of the Court (uncredited) | Lady of the Court (uncredited) |
 Maris WrixonLady of the Court (uncredited) | Lady of the Court (uncredited) |
