 Warner BaxterDr. Samuel Alexander Mudd | Dr. Samuel Alexander Mudd |
 Gloria StuartMrs. Peggy Mudd | Mrs. Peggy Mudd |
 Claude GillingwaterColonel Dyer | Colonel Dyer |
 Arthur ByronMr. Erickson | Mr. Erickson |
 O. P. HeggieDr. Mac Intyre | Dr. Mac Intyre |
 Harry CareyCommandant | Commandant |
 Francis FordCorporal O'Toole | Corporal O'Toole |
 John McGuireLieutenant Lovett | Lieutenant Lovett |
 Francis McDonaldJohn Wilkes Booth | John Wilkes Booth |
 Douglas WoodGeneral Ewing | General Ewing |
 John CarradineSergeant Rankin | Sergeant Rankin |
| JJoyce KayMartha Mudd | Martha Mudd |
 Fred Kohler Jr.Sergeant Cooper | Sergeant Cooper |
 Ernest WhitmanBuck | Buck |
 Paul FixDavid Herold | David Herold |
 Frank ShannonMr. Holt | Mr. Holt |
 Frank McGlynn Sr.Abraham Lincoln | Abraham Lincoln |
| LLeila McIntyreMrs. Abraham Lincoln | Mrs. Abraham Lincoln |
 Etta McDanielAunt Rosabelle | Aunt Rosabelle |
 J.M. KerriganJudge Maiben | Judge Maiben |
 Arthur LoftCarpet Bagger | Carpet Bagger |
 Paul McVeyGeneral Hunter | General Hunter |
 Maurice MurphyOrderly | Orderly |
 Matthew BeardBoy at Dr. Mudd's Door (uncredited) | Boy at Dr. Mudd's Door (uncredited) |
 Frank BakerUndetermined Secondary Role (uncredited) | Undetermined Secondary Role (uncredited) |
 Stanley BlystoneBoy Seeking Dr. Mudd (uncredited) | Boy Seeking Dr. Mudd (uncredited) |
| SStanley BordagarayUndetermined Secondary Role (uncredited) | Undetermined Secondary Role (uncredited) |
 Whitney BourneUndetermined Secondary Role (uncredited) | Undetermined Secondary Role (uncredited) |
 Lester DorrGeorge A. Atzerodt (uncredited) | George A. Atzerodt (uncredited) |
 Robert DudleyDruggist at Trial (uncredited) | Druggist at Trial (uncredited) |
