 Anika Noni RoseTiana (voice) | Tiana (voice) |
 Bruno CamposPrince Naveen (voice) | Prince Naveen (voice) |
 Jim CummingsRay (voice) | Ray (voice) |
 Michael-Leon WooleyLouis (voice) | Louis (voice) |
 Keith DavidDr. Facilier (voice) | Dr. Facilier (voice) |
 Jennifer CodyCharlotte (voice) | Charlotte (voice) |
 Peter BartlettLawrence (voice) | Lawrence (voice) |
 Jenifer LewisMama Odie (voice) | Mama Odie (voice) |
 Oprah WinfreyEudora (voice) | Eudora (voice) |
 John Goodman"Big Daddy" La Bouff (voice) | "Big Daddy" La Bouff (voice) |
 Terrence HowardJames (voice) | James (voice) |
 Ritchie MontgomeryReggie (voice) | Reggie (voice) |
 Don HallDarnell (voice) | Darnell (voice) |
 Paul BriggsTwo Fingers (voice) | Two Fingers (voice) |
 Jerry KernionMr. Henry Fenner (voice) | Mr. Henry Fenner (voice) |
 Corey BurtonMr. Harvey Fenner (voice) | Mr. Harvey Fenner (voice) |
 Elizabeth DampierYoung Tiana (voice) | Young Tiana (voice) |
 Breanna BrooksYoung Charlotte (voice) | Young Charlotte (voice) |
 Michael ColyarBuford (voice) | Buford (voice) |
 Emeril LagasseMarlon the Gator (voice) | Marlon the Gator (voice) |
 Kevin Michael RichardsonIan the Gator (voice) | Ian the Gator (voice) |
 Randy NewmanCousin Randy (voice) | Cousin Randy (voice) |
 Terence BlanchardLouis' Trumpet Playing (voice) | Louis' Trumpet Playing (voice) |
 Mick WingertTravis (voice) | Travis (voice) |
| SSeth R. WilliamsonPrince Ralphie (voice) | Prince Ralphie (voice) |
| SShane R. WilliamsonTeenage Kid (voice) | Teenage Kid (voice) |
 Phil ProctorCajun Firefly (voice) | Cajun Firefly (voice) |
 Peter RenadayAdditional Voices (voice) | Additional Voices (voice) |
