Due to an experimental vaccine, Dr. Robert Neville is the only human survivor of an apocalyptic war waged with biological weapons. Besides him, only a few hundred deformed, nocturnal people remain; sensitive to light, and homicidally psychotic.
| Actor | Role |
|---|---|
 Charlton HestonRobert Neville | Robert Neville |
 Anthony ZerbeMatthias | Matthias |
 Rosalind CashLisa | Lisa |
 Paul KosloDutch | Dutch |
 Eric LaneuvilleRichie | Richie |
 Lincoln KilpatrickZachary | Zachary |
 Anna AriesWoman in Cemetery Crypt | Woman in Cemetery Crypt |
 Brian TochiTommy | Tommy |
 DeVeren BookwalterFamily Member | Family Member |
 John DierkesFamily Member | Family Member |
| JJill GiraldiLittle Girl | Little Girl |
| MMonika HenreidFamily Member | Family Member |
| LLinda RedfearnFamily Member | Family Member |
| FForrest WoodFamily Member | Family Member |
| SSteve GoldsteinLast Boy (uncredited) | Last Boy (uncredited) |