Having briefly abandoned his standard "Nevada Jack McKenzie" characterization in Flame of the West, cowboy star Johnny Mack Brown was back as Nevada Jack in Monogram's The Lost Trail. Vowing to bring in a gang of stagecoach outlaws, Nevada redoubles his efforts when he learns that the owner of the stagecoach line is pretty Jane Burns (Jennifer Holt).
| Actor | Role |
|---|---|
 Johnny Mack BrownMarshal 'Nevada Jack' McKenzie | Marshal 'Nevada Jack' McKenzie |
 Raymond HattonMarshal Sandy 'Trigger' Hopkins | Marshal Sandy 'Trigger' Hopkins |
 Jennifer HoltJane Burns | Jane Burns |
 Riley HillNed Turner | Ned Turner |
 Kenneth MacDonaldJohn Corbett | John Corbett |
 Eddie ParkerHenchman Bill | Henchman Bill |
 John InceBailey (Stable Owner) | Bailey (Stable Owner) |
 Frank LaRueMine Owner Jones | Mine Owner Jones |
 Steve ClarkMine Owner Mason | Mine Owner Mason |
 Milburn MoranteZeke (Handyman) | Zeke (Handyman) |
 Lynton BrentHall (Mine Owner) (as Lynton W. Brent) | Hall (Mine Owner) (as Lynton W. Brent) |
| FFrank McCarrollHenchman Joe | Henchman Joe |
 Dick DickinsonBartender Ed | Bartender Ed |
| HHenry VroomHenchman Tom | Henchman Tom |