 Will ArnettBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Michael CeraRobin / Dick Grayson (voice) | Robin / Dick Grayson (voice) |
 Rosario DawsonBatgirl / Barbara Gordon (voice) | Batgirl / Barbara Gordon (voice) |
 Ralph FiennesAlfred Pennyworth (voice) | Alfred Pennyworth (voice) |
 Zach GalifianakisJoker (voice) | Joker (voice) |
 Jenny SlateHarley Quinn (voice) | Harley Quinn (voice) |
 Jason MantzoukasScarecrow (voice) | Scarecrow (voice) |
 Conan O'BrienThe Riddler (voice) | The Riddler (voice) |
 Doug BensonBane (voice) | Bane (voice) |
 Billy Dee WilliamsTwo-Face (voice) | Two-Face (voice) |
 Zoë KravitzCatwoman (voice) | Catwoman (voice) |
 Kate MicucciClayface (voice) | Clayface (voice) |
 Riki LindhomePoison Ivy (voice) | Poison Ivy (voice) |
 Suzy Eddie IzzardVoldemort (voice) | Voldemort (voice) |
 Seth GreenKing Kong (voice) | King Kong (voice) |
 Jemaine ClementSauron (voice) | Sauron (voice) |
 Ellie KemperPhyllis (voice) | Phyllis (voice) |
 Channing TatumSuperman (voice) | Superman (voice) |
 Jonah HillGreen Lantern (voice) | Green Lantern (voice) |
 Adam DevineThe Flash (voice) | The Flash (voice) |
 Héctor ElizondoJim Gordon (voice) | Jim Gordon (voice) |
 Mariah CareyMayor McCaskill (voice) | Mayor McCaskill (voice) |
 Lauren WhiteChief O'Hara / Medusa / Additional Voices (voice) | Chief O'Hara / Medusa / Additional Voices (voice) |
 David BurrowsAnchorman Phil (voice) | Anchorman Phil (voice) |
 Laura KightlingerReporter Pippa (voice) | Reporter Pippa (voice) |
 Brent MusburgerReporter #1 (voice) | Reporter #1 (voice) |
 Ralph GarmanReporter #2 (voice) | Reporter #2 (voice) |
 Chris HardwickReporter #3 (voice) | Reporter #3 (voice) |
| TTodd HansenCaptain Dale (voice) | Captain Dale (voice) |
 Chris McKayPilot Bill / Additional Voices (voice) | Pilot Bill / Additional Voices (voice) |
