 Elizabeth HolmesSelf - CEO and Founder of Theranos (archive footage) | Self - CEO and Founder of Theranos (archive footage) |
 Alex GibneySelf - Narrator / Interviewer | Self - Narrator / Interviewer |
 Dan ArielySelf - Behavioral Economist | Self - Behavioral Economist |
 Roger ParloffSelf - Fortune | Self - Fortune |
| KKen AulettaSelf - The New Yorker | Self - The New Yorker |
| EErika CheungSelf - Lab Associate | Self - Lab Associate |
| CCheryl GafnerSelf - Receptionist | Self - Receptionist |
| DDave PhilippidesSelf - Engineer | Self - Engineer |
| DDouglas MatjeSelf - Biochemist | Self - Biochemist |
| RRyan WistortSelf - Research & Development | Self - Research & Development |
| TTony NugentSelf - Vice President, Product Development | Self - Vice President, Product Development |
 Phyllis GardnerSelf - Professor of Medicine, Stanford University | Self - Professor of Medicine, Stanford University |
| CChanning RobertsonSelf - Theranos Advisor | Self - Theranos Advisor |
| DDon LucasSelf - Investor | Self - Investor |
| TTim DraperSelf - Investor | Self - Investor |
| TTyler ShultzSelf - Research Engineer | Self - Research Engineer |
| RRochelle GibbonsSelf - Ian Gibbons' Wife | Self - Ian Gibbons' Wife |
| MMatt HernanSelf - Facilities Coordinator | Self - Facilities Coordinator |
| PPatrick O'NeillSelf - Chief Creative Officer | Self - Chief Creative Officer |
 Errol MorrisSelf | Self |
| SStephanie SeitzSelf | Self |
| SSerena StewartSelf - Phlebotomy Trainer | Self - Phlebotomy Trainer |
| JJohn CarreyrouSelf - The Wall Street Journal, Author, Bad Blood | Self - The Wall Street Journal, Author, Bad Blood |
 David Boies IIISelf - Boies, Schiller & Flexner LLP | Self - Boies, Schiller & Flexner LLP |
| RRamesh BalwaniSelf - President and Chief Operating Officer (archive footage) | Self - President and Chief Operating Officer (archive footage) |
| PPattie SellersSelf (archive footage) | Self (archive footage) |
 Charlie RoseSelf (archive footage) | Self (archive footage) |
 Thomas A. EdisonSelf (archive footage) | Self (archive footage) |
| GGeorge ShultzSelf (archive footage) | Self (archive footage) |
 James MattisSelf (archive footage) | Self (archive footage) |
