 Richard GreeneSir Henry Baskerville | Sir Henry Baskerville |
 Basil RathboneSherlock Holmes | Sherlock Holmes |
 Wendy BarrieBeryl Stapleton | Beryl Stapleton |
 Nigel BruceDoctor John H. Watson | Doctor John H. Watson |
 Lionel AtwillDr. James Mortimer | Dr. James Mortimer |
 John CarradineBarryman | Barryman |
 Barlowe BorlandFrankland | Frankland |
 Beryl MercerMrs. Jennifer Mortimer | Mrs. Jennifer Mortimer |
 Morton LowryJohn 'Jack' Stapleton | John 'Jack' Stapleton |
 Ralph ForbesSir Hugo Baskerville | Sir Hugo Baskerville |
 E. E. CliveLondon Cabbie John Clayton | London Cabbie John Clayton |
 Eily MalyonMrs. Barryman | Mrs. Barryman |
| LLionel PapeThe Coroner | The Coroner |
 Nigel De BrulierThe Notting Hill Murderer | The Notting Hill Murderer |
 Mary GordonMrs. Hudson | Mrs. Hudson |
| IIan MaclarenSir Charles Baskerville | Sir Charles Baskerville |
 Ruth TerryBetsy Ann | Betsy Ann |
 Mary YoungBetsy Ann's Mother (uncredited) | Betsy Ann's Mother (uncredited) |
| KKenneth HunterShip's Officer (uncredited) | Ship's Officer (uncredited) |
| VVesey O'DavorenShip's Steward (uncredited) | Ship's Steward (uncredited) |
| JJohn Graham SpaceyShip's Porter (uncredited) | Ship's Porter (uncredited) |
| DDavid ThursbyOpen Carriage Driver (uncredited) | Open Carriage Driver (uncredited) |
 Peter WillesRoderick | Roderick |
 Leonard CareyHugo's Servant (uncredited) | Hugo's Servant (uncredited) |
 Rita PageChambermaid (uncredited) | Chambermaid (uncredited) |
| JJack Egger(uncredited) | (uncredited) |
 Frank McLureShip Passenger (uncredited) | Ship Passenger (uncredited) |
| BBrandon BeachShip Passenger (uncredited) | Ship Passenger (uncredited) |
| BBlitzenThe Hound (as Chief) | The Hound (as Chief) |
| JJames CarlisleShip Passenger (uncredited) | Ship Passenger (uncredited) |
