 John WayneCol John Marlowe | Col John Marlowe |
 William HoldenMajor 'Hank' Kendall | Major 'Hank' Kendall |
 Constance TowersMiss Hannah Hunter | Miss Hannah Hunter |
 Judson PrattSgt Major Kirby | Sgt Major Kirby |
 Hoot GibsonSgt. Brown | Sgt. Brown |
 Ken CurtisCpl. Wilkie | Cpl. Wilkie |
 Willis BoucheyCol. Phil Secord | Col. Phil Secord |
 Bing RussellDunker, Yankee Soldier Amputee | Dunker, Yankee Soldier Amputee |
 O.Z. WhiteheadOtis 'Hoppy' Hopkins | Otis 'Hoppy' Hopkins |
 Hank WordenDeacon Clump | Deacon Clump |
 Chuck HaywardUnion Captain | Union Captain |
 Denver PyleJackie Jo | Jackie Jo |
 Strother MartinVirgil | Virgil |
 Basil RuysdaelThe Reverend (Jefferson Military Academy) | The Reverend (Jefferson Military Academy) |
 Carleton YoungCol. Jonathan Miles, CSA | Col. Jonathan Miles, CSA |
 William LeslieGray | Gray |
 William HenryCaptain | Captain |
 Walter ReedUnion Officer | Union Officer |
 Anna LeeMrs. Buford | Mrs. Buford |
 William ForrestGen. Steve Hurlburt | Gen. Steve Hurlburt |
 Ron HagerthyBugler | Bugler |
 Russell SimpsonHenry Goodbody | Henry Goodbody |
| AAlthea GibsonLukey | Lukey |
 Gertrude AstorTownswoman (uncredited) | Townswoman (uncredited) |
 Bill BorzageTownsman (uncredited) | Townsman (uncredited) |
| DDanny BorzageOld Soldier in Line (uncredited) | Old Soldier in Line (uncredited) |
 John FordNed (uncredited) | Ned (uncredited) |
 Jack PennickMitch (uncredited) | Mitch (uncredited) |
| JJan StineHoskins - Dying Soldier (uncredited) | Hoskins - Dying Soldier (uncredited) |
| SSam HarrisMajor (uncredited) | Major (uncredited) |
