 Chris PrattPeter Quill / Star-Lord | Peter Quill / Star-Lord |
 Dave BautistaDrax | Drax |
 Karen GillanNebula | Nebula |
 Pom KlementieffMantis | Mantis |
 Vin DieselGroot (voice) | Groot (voice) |
 Bradley CooperRocket (voice) | Rocket (voice) |
 Sean GunnKraglin | Kraglin |
 Rhett MillerBzermikitokolok | Bzermikitokolok |
 Murry HammondAlien Band (Kortolbookalia) | Alien Band (Kortolbookalia) |
| KKen BetheaAlien Band (Sliyavastojoo) | Alien Band (Sliyavastojoo) |
| PPhilip PeeplesAlien Band (Phloko) | Alien Band (Phloko) |
 Michael RookerYondu | Yondu |
 Kevin BaconKevin Bacon | Kevin Bacon |
 Luke KleinYoung Peter Quill | Young Peter Quill |
 Maria BakalovaCosmo the Dog (voice) | Cosmo the Dog (voice) |
 Kyra SedgwickKyra Sedgwick (voice) | Kyra Sedgwick (voice) |
| SSi ChenSobbing Woman | Sobbing Woman |
| DDon McLeodStatue Guy | Statue Guy |
 Thomas McNamara'Captain America' | 'Captain America' |
 Daniel BernhardtGobot | Gobot |
 Michelle GunnTourist Mom | Tourist Mom |
 Flula BorgBartender | Bartender |
| MMatthew WithersHandsome Man | Handsome Man |
 Rusty SchwimmerSara | Sara |
| HHelene D'AuriaDispatcher (voice) | Dispatcher (voice) |
 Tinashe KajeseOfficer Fitzgibbon | Officer Fitzgibbon |
| BBarry CurtisOfficer R. Bobbitt | Officer R. Bobbitt |
 Rachel LuttrellUpside-Down Cop | Upside-Down Cop |
 Giovannie CruzOrloni Peddler | Orloni Peddler |
 Stephen BlackehartSteemie | Steemie |
