 Barrie InghamBasil of Baker Street / Bartholomew (voice) | Basil of Baker Street / Bartholomew (voice) |
 Vincent PriceProfessor Ratigan (voice) | Professor Ratigan (voice) |
 Val BettinDr. David Q. Dawson / Thug Guard (voice) | Dr. David Q. Dawson / Thug Guard (voice) |
| SSusanne PollatschekOlivia Flaversham (voice) | Olivia Flaversham (voice) |
 Candy CandidoFidget (voice) | Fidget (voice) |
 Diana ChesneyMrs. Judson (voice) | Mrs. Judson (voice) |
 Eve BrennerThe Mouse Queen (voice) | The Mouse Queen (voice) |
 Alan YoungMr. Flaversham (voice) | Mr. Flaversham (voice) |
 Basil RathboneSherlock Holmes (voice) | Sherlock Holmes (voice) |
| EEllen FitzhughBar Maid (voice) | Bar Maid (voice) |
 Walker EdmistonCitizen / Thug Guard (voice) | Citizen / Thug Guard (voice) |
 Wayne AllwineThug Guard (voice) | Thug Guard (voice) |
 Tony AnselmoThug Guard (voice) | Thug Guard (voice) |
 Frank WelkerToby the Dog / Felicia the Cat (voice) (uncredited) | Toby the Dog / Felicia the Cat (voice) (uncredited) |
 Laurie MainDr. Watson (archive sound) | Dr. Watson (archive sound) |
 Shani WallisLady Mouse (voice) | Lady Mouse (voice) |
 Melissa ManchesterMiss Kitty Mouse (voice) (uncredited) | Miss Kitty Mouse (voice) (uncredited) |
