 Cedric HardwickeDr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image) | Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image) |
 Ralph BellamyErik Ernst | Erik Ernst |
 Lionel AtwillDr. Bohmer | Dr. Bohmer |
 Bela LugosiYgor | Ygor |
 Evelyn AnkersElsa Frankenstein | Elsa Frankenstein |
 Lon Chaney Jr.The Frankenstein Monster | The Frankenstein Monster |
| JJanet Ann GallowCloestine Hussman | Cloestine Hussman |
 Barton YarboroughDr. Kettering | Dr. Kettering |
 Doris LloydMartha the Housekeeper | Martha the Housekeeper |
 Leyland HodgsonChief Constable | Chief Constable |
 Olaf HyttenAdolph Hussman | Adolph Hussman |
 Holmes HerbertMagistrate | Magistrate |
 Richard AlexanderVillager at Council Meeting (uncredited) | Villager at Council Meeting (uncredited) |
 Lionel BelmoreCouncilman (uncredited) | Councilman (uncredited) |
 Chet BrandenburgVillager (uncredited) | Villager (uncredited) |
 Colin CliveDr. Henry Frankenstein (flashback) (uncredited) | Dr. Henry Frankenstein (flashback) (uncredited) |
 Harry CordingMob Leader (uncredited) | Mob Leader (uncredited) |
 George EldredgeConstable (uncredited) | Constable (uncredited) |
 Dwight FryeVillager at Meeting / Grave Robber (flashback) (uncredited) | Villager at Meeting / Grave Robber (flashback) (uncredited) |
 Lawrence GrantMayor (uncredited) | Mayor (uncredited) |
 Otto HoffmanVillager (uncredited) | Villager (uncredited) |
 Brandon HurstHans, Villager at Council Meeting (uncredited) | Hans, Villager at Council Meeting (uncredited) |
 Michael MarkCouncilman (uncredited) | Councilman (uncredited) |
| JJimmy PhillipsBoy Playing Indian (uncredited) | Boy Playing Indian (uncredited) |
 William SmithBoy (uncredited) | Boy (uncredited) |
 Ernie StantonConstable (uncredited) | Constable (uncredited) |
 Julius TannenSektal (uncredited) | Sektal (uncredited) |
 Harry TenbrookVillager at Hearing (uncredited) | Villager at Hearing (uncredited) |
| GGlen WaltersMother of Hungry Children (uncredited) | Mother of Hungry Children (uncredited) |
 Janet WarrenGoose-herd (uncredited) | Goose-herd (uncredited) |
