 Ted DansonNarrator (voice) | Narrator (voice) |
 Ben BradshawSelf - UK Fisheries Minister 2003-2007 | Self - UK Fisheries Minister 2003-2007 |
| RRoberto Mielgo BragazziSelf - Former Tuna Farmer | Self - Former Tuna Farmer |
| CCharles CloverSelf - Author, "The End of the Line" | Self - Author, "The End of the Line" |
| JJohn CrosbieSelf - Fisheries Minister, Canada (archive footage) | Self - Fisheries Minister, Canada (archive footage) |
| HHaidar El AliSelf - Diver, Senegal | Self - Diver, Senegal |
| RRay HilbornSelf - University of Washington | Self - University of Washington |
| JJeffrey HutchingsSelf - Dalhousie University, Canada | Self - Dalhousie University, Canada |
 Mélanie LaurentNarrator for France (voice) | Narrator for France (voice) |
| MManolo Pacheco LuisSelf - Fisherman, Straits of Gibraltar | Self - Fisherman, Straits of Gibraltar |
| PPatricia MajlufSelf - Cayetano Heredia University, Peru | Self - Cayetano Heredia University, Peru |
| JJohn T. MaxwellSelf - American Television Chef (archive footage) | Self - American Television Chef (archive footage) |
| AAdama MbergaulSelf - Fisherman, Senegal | Self - Fisherman, Senegal |
| HHardy MckinneySelf - Fisherman, South Andros, Bahamas | Self - Fisherman, South Andros, Bahamas |
| MMasanori MiyaharaSelf - Fisheries Agency of Japan | Self - Fisheries Agency of Japan |
| MMatthew MoirSelf - North Pacific Seafoods, Alaska | Self - North Pacific Seafoods, Alaska |
 Brian MulroneySelf - Canadian Prime Minister 1984-1993 (archive footage) | Self - Canadian Prime Minister 1984-1993 (archive footage) |
| RRichie NotaSelf - Managing Partner, Nobu Restaurants | Self - Managing Partner, Nobu Restaurants |
 Jamie OliverSelf - British Chef (archive footage) | Self - British Chef (archive footage) |
| SStephen R. PalumbiSelf - Stanford University | Self - Stanford University |
| DDaniel PaulySelf - University of British Columbia | Self - University of British Columbia |
| PPete PetersenSelf - University of North Carolina | Self - University of North Carolina |
| CCallum RobertsSelf - York University, UK | Self - York University, UK |
| YYvonne SadovySelf - University of Hong Kong | Self - University of Hong Kong |
| TTed StevensSelf - Senator, Alaska (archive footage) | Self - Senator, Alaska (archive footage) |
| RRashid SumailaSelf - University of British Columbia | Self - University of British Columbia |
| SSerge TudelaSelf - Bluefin Tuna Expert World Wildlife Foundation | Self - Bluefin Tuna Expert World Wildlife Foundation |
| BBoris WormSelf - Dalhousie University Canada | Self - Dalhousie University Canada |
