 James MarsdenTim (voice) | Tim (voice) |
 Alec BaldwinBoss Baby (Ted) (voice) | Boss Baby (Ted) (voice) |
 Amy SedarisTina (voice) | Tina (voice) |
 Ariana GreenblattTabitha (voice) | Tabitha (voice) |
 Jeff GoldblumDr. Armstrong (voice) | Dr. Armstrong (voice) |
 Eva LongoriaCarol (voice) | Carol (voice) |
 James McGrathWizzie (voice) | Wizzie (voice) |
 Jimmy KimmelGrandpa (Ted Sr.) (voice) | Grandpa (Ted Sr.) (voice) |
 Lisa KudrowGrandma (Janice) (voice) | Grandma (Janice) (voice) |
 Raphael AlejandroNathan / Lead Baby Ninja (voice) | Nathan / Lead Baby Ninja (voice) |
 Serenity Reign BrownMeghan (voice) | Meghan (voice) |
 David SorenJimbo (voice) | Jimbo (voice) |
| NNicholas GistThe Triplets (voice) | The Triplets (voice) |
 James RyanStory Bear (Connie) (voice) | Story Bear (Connie) (voice) |
 David P. SmithMovie Patron (voice) | Movie Patron (voice) |
| NNova ReedLittle Bo Peep Baby (voice) | Little Bo Peep Baby (voice) |
| MMolly K. GrayCreepy Girl (voice) | Creepy Girl (voice) |
| AAshlyn Lundahl'No' Girl (voice) | 'No' Girl (voice) |
| DDave NeedhamGlue Baby (voice) | Glue Baby (voice) |
 Tom McGrathDr. Tiffany Hamilton (voice) | Dr. Tiffany Hamilton (voice) |
 Reyn DoiBox Kid (voice) | Box Kid (voice) |
| CCollin ErkerTime Out Kid (voice) | Time Out Kid (voice) |
 Walt DohrnBaby Bouncer (voice) | Baby Bouncer (voice) |
| AApril LawrenceAcorn Center Computer (voice) | Acorn Center Computer (voice) |
 Miles BakshiYoung Tim Voice-Over (voice) | Young Tim Voice-Over (voice) |
| KKristin LowePageant Mom 1 (voice) | Pageant Mom 1 (voice) |
| MMarjorie CohnPageant Mom 2 (voice) | Pageant Mom 2 (voice) |
| CConnor AlbrechtDreidel Kid (voice) | Dreidel Kid (voice) |
