 Miles BakshiTim (voice) | Tim (voice) |
 Alec BaldwinBoss Baby (voice) | Boss Baby (voice) |
 Jimmy KimmelDad (voice) | Dad (voice) |
 Lisa KudrowMom (voice) | Mom (voice) |
 Steve BuscemiFrancis Francis (voice) | Francis Francis (voice) |
 Tobey MaguireAdult Tim / Narrator (voice) | Adult Tim / Narrator (voice) |
 James McGrathWizzie / Elvis Impersonator (voice) | Wizzie / Elvis Impersonator (voice) |
 Conrad VernonEugene (voice) | Eugene (voice) |
 ViviAnn YeeStaci (voice) | Staci (voice) |
 Eric Bell Jr.Triplets (voice) | Triplets (voice) |
 David SorenJimbo (voice) | Jimbo (voice) |
| EEdie MirmanBig Boss Baby (voice) | Big Boss Baby (voice) |
 James RyanStory Bear (voice) | Story Bear (voice) |
 Walt DohrnPhotographer (voice) | Photographer (voice) |
| JJules WinterCrying Boy / Little Boy / Boy (voice) | Crying Boy / Little Boy / Boy (voice) |
| NNina Zoe BakshiTim's Daughter / Girl / Little Girl (voice) | Tim's Daughter / Girl / Little Girl (voice) |
 Tom McGrathTV Chef (voice) | TV Chef (voice) |
 Brian HopkinsAirport Security Guard (voice) | Airport Security Guard (voice) |
| GGlenn HarmonAirport Announcer (voice) | Airport Announcer (voice) |
 Joseph IzzoElvis Impersonators (voice) | Elvis Impersonators (voice) |
 Chris MillerCaptain Ross (voice) | Captain Ross (voice) |
| AAndrea Montana KnollFlight Attendant (voice) | Flight Attendant (voice) |
| CChloe AlbrechtHazmat Baby (voice) | Hazmat Baby (voice) |
