A seductive and ruthless spy in the service of Germany steals state secrets- including the formula for poison gas-from England and France during World War I. British intelligence, in turn, tries to hunt her down.
| Actor | Role |
|---|---|
 Suzy KendallFräulein Doktor | Fräulein Doktor |
 Kenneth MoreColonel Foreman | Colonel Foreman |
 Nigel GreenColonel Mathesius | Colonel Mathesius |
 Alexander KnoxGeneral Peronne | General Peronne |
 Olivera KatarinaMarchioness de Haro | Marchioness de Haro |
 CapucineDr. Saforet | Dr. Saforet |
 James BoothMeyer | Meyer |
 Andreina PaulDona Elena de Rivas | Dona Elena de Rivas |
 Silvia MontiMargarita | Margarita |
 Virginia BellDona Julia | Dona Julia |
 Giancarlo GianniniHans Ruppert | Hans Ruppert |
 Mario NovelliSgt. Otto Latemar | Sgt. Otto Latemar |
 Roberto BisaccoSchell | Schell |
| MMilivoje 'Mavid' PopovićChaplain | Chaplain |
 Janez VrhovecBelgian Colonel | Belgian Colonel |
 Michael ElphickTom | Tom |