The film spans from Hepburn's early childhood to the 1950s which details her life as a Dutch ballerina, coming to grips with her parents' divorce, and enduring life in the Nazi-occupied Netherlands during World War II. She then settles in the U.S. where she succeeds in making it big as a movie actress, in such movies as Breakfast at Tiffany's.
| Actor | Role |
|---|---|
 Jennifer Love HewittAudrey Hepburn | Audrey Hepburn |
 Frances FisherElla van Heemstra | Ella van Heemstra |
 Keir DulleaJoseph Hepburn | Joseph Hepburn |
 Gabriel MachtWilliam Holden | William Holden |
 Peter GilesJames Hanson | James Hanson |
 Emmy RossumYoung Audrey Hepburn (ages 12-16) | Young Audrey Hepburn (ages 12-16) |
 Eric McCormackMel Ferrer | Mel Ferrer |
 Seana KofoedKay Kendall | Kay Kendall |
 Michael J. BurgTruman Capote | Truman Capote |
 Stéphane ArchambaultMarcel | Marcel |
 Joan CopelandCathleen Nesbitt | Cathleen Nesbitt |
 Marcel JeanninGivenchy | Givenchy |
 Adam MacDonaldNick Dana | Nick Dana |
| MMark CamachoTiffany's Cab Driver | Tiffany's Cab Driver |
 Sarah HylandAudrey Hepburn (at 8 years old) | Audrey Hepburn (at 8 years old) |
 Vlasta VranaUncle Willem | Uncle Willem |
 Barry JulienSkip | Skip |
 Lise RoySister Luc | Sister Luc |
 Catherine ColveyMme. Rombert | Mme. Rombert |
 Bruce DinsmoreBlake Edwards | Blake Edwards |
 Dorothée BerrymanElla's Friend | Ella's Friend |
 Martin WatierGaston | Gaston |