 Zach Tyler EisenLucas Nickle (voice) | Lucas Nickle (voice) |
 Julia RobertsHova (voice) | Hova (voice) |
 Meryl StreepQueen (voice) | Queen (voice) |
 Nicolas CageZoc (voice) | Zoc (voice) |
 Paul GiamattiStan Beals (voice) | Stan Beals (voice) |
 Bruce CampbellFugax (voice) | Fugax (voice) |
 Regina KingKreela (voice) | Kreela (voice) |
 Lily TomlinMommo (voice) | Mommo (voice) |
 Larry MillerFred Nickle (voice) | Fred Nickle (voice) |
 Cheri OteriDoreen Nickle (voice) | Doreen Nickle (voice) |
 Ricardo MontalbanHead of Council (voice) | Head of Council (voice) |
 Myles JeffreySteve (voice) | Steve (voice) |
 Creagen DowMullet Boy (voice) | Mullet Boy (voice) |
 Frank WelkerSpindle / Frog / Caterpillar (voice) | Spindle / Frog / Caterpillar (voice) |
 Mark DeCarloFly (voice) | Fly (voice) |
 Jake T. AustinNicky (voice) | Nicky (voice) |
 Allison MackTiffany Nickle (voice) | Tiffany Nickle (voice) |
 S. Scott BullockGlow Worm / Wasp Survivor (voice) | Glow Worm / Wasp Survivor (voice) |
 Paul GreenbergSleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice) | Sleeping Ant #1 / Head Lice #1 / Head Lice #2 / Head Lice #3 / Head Lice #4 / Brett (voice) |
 Rob PaulsenBeetle (voice) | Beetle (voice) |
 Nicole SullivanAnt #9 (voice) | Ant #9 (voice) |
 Don FryeSoldier Ant (voice) | Soldier Ant (voice) |
 Larry CedarAnt #8 (voice) | Ant #8 (voice) |
 David KayeSleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice) | Sleeping Ant #2 / Guard Ant #3 / Wrangler Ant (voice) |
| AAlfred JacksonSlacker Ant (voice) | Slacker Ant (voice) |
| KKen MitchroneyAnt #17 / Guard Ant #2 / Wasp #8 (voice) | Ant #17 / Guard Ant #2 / Wasp #8 (voice) |
 Denzel WhitakerAlbert (voice) | Albert (voice) |
| CCasey MasamitsuShigeko (voice) | Shigeko (voice) |
| BBryan FabianFernando (voice) | Fernando (voice) |
 Jake GoldbergHelmet Boy / Nob / Red Teammate #1 (voice) | Helmet Boy / Nob / Red Teammate #1 (voice) |
