 Basil RathboneSherlock Holmes | Sherlock Holmes |
 Nigel BruceDr. Watson | Dr. Watson |
 Ida LupinoAnn Brandon | Ann Brandon |
 Alan MarshalJerrold Hunter | Jerrold Hunter |
 Terry KilburnBilly | Billy |
 George ZuccoProfessor Moriarty | Professor Moriarty |
 Henry StephensonSir Ronald Ramsgate | Sir Ronald Ramsgate |
 E. E. CliveInspector Bristol | Inspector Bristol |
 Arthur HohlHenchman Bassick | Henchman Bassick |
 May BeattyMrs. Jameson, Brandon Housekeeper | Mrs. Jameson, Brandon Housekeeper |
 Peter WillesLloyd Brandon | Lloyd Brandon |
 Mary GordonMrs. Hudson | Mrs. Hudson |
 Holmes HerbertJustice of the Court | Justice of the Court |
 George RegasHenchman Mateo | Henchman Mateo |
 Mary ForbesLady Conyngham | Lady Conyngham |
| FFrank DawsonDawes, Moriarty's servant | Dawes, Moriarty's servant |
 William AustinInquisitive Stranger | Inquisitive Stranger |
| AAnthony Kemble-CooperTony Conyngham | Tony Conyngham |
 Leonard MudieBarrows - Moriarty's Attorney (uncredited) | Barrows - Moriarty's Attorney (uncredited) |
| RRobert NobelForeman of the Jury (uncredited) | Foreman of the Jury (uncredited) |
 Ivan F. SimpsonGates - Trial Prosecutor (uncredited) | Gates - Trial Prosecutor (uncredited) |
| NNeil FitzgeraldClerk of the Court (uncredited) | Clerk of the Court (uncredited) |
 Frank BakerTompkins (uncredited) | Tompkins (uncredited) |
 Denis GreeneDenis Green (uncredited) | Denis Green (uncredited) |
| RRobert CoryTower Sentry (uncredited) | Tower Sentry (uncredited) |
| GGordon HartGuard (uncredited) | Guard (uncredited) |
| KKeith HitchcockConstable (uncredited) | Constable (uncredited) |
| IIvo HendersonBobby (uncredited) | Bobby (uncredited) |
 Leyland HodgsonBobby (uncredited) | Bobby (uncredited) |
 Boyd IrwinBobby (uncredited) | Bobby (uncredited) |
