 Basil RathboneSherlock Holmes | Sherlock Holmes |
 Nigel BruceDr. John H. Watson | Dr. John H. Watson |
 Alan MowbrayMajor Duncan-Bleek | Major Duncan-Bleek |
 Dennis HoeyInspector Lestrade | Inspector Lestrade |
 Renee GodfreyVivian Vedder | Vivian Vedder |
 Frederick WorlockProf. William Kilbane | Prof. William Kilbane |
 Mary ForbesLady Margaret Carstairs | Lady Margaret Carstairs |
 Skelton KnaggsSands - Moran's Accomplice | Sands - Moran's Accomplice |
 Billy BevanConductor Taking Tickets | Conductor Taking Tickets |
| GGeoffrey SteeleRoland Carstairs | Roland Carstairs |
| JJanet MurdochMrs. Shallcross (uncredited) | Mrs. Shallcross (uncredited) |
 Gerald HamerAlf Shallcross (uncredited) | Alf Shallcross (uncredited) |
 Leyland HodgsonSenior Conductor (uncredited) | Senior Conductor (uncredited) |
 Harry CordingMock the Undertaker (uncredited) | Mock the Undertaker (uncredited) |
| CCharles KnightBaggage Car Guard (uncredited) | Baggage Car Guard (uncredited) |
| BBoyd Davis"Inspector MacDonald" (uncredited) | "Inspector MacDonald" (uncredited) |
| BBobby WisslerMock Jr. (uncredited) | Mock Jr. (uncredited) |
| GGilbert AllenDining Car Steward (uncredited) | Dining Car Steward (uncredited) |
 C. Aubrey SmithElderly gentleman on train station (uncredited) | Elderly gentleman on train station (uncredited) |
