Donnie, Raph, Leo, and Mikey are back for their next adventure with Master Splinter, the Mutants, April, and a new villain they have to face: The Shredder.
| Actor | Role |
|---|---|
 Micah AbbeyDonatello (voice) | Donatello (voice) |
 Nicolas CantuLeonardo (voice) | Leonardo (voice) |
 Brady NoonRaphael (voice) | Raphael (voice) |
 Shamon Brown Jr.Michelangelo (voice) | Michelangelo (voice) |
 Jackie ChanSplinter (voice) | Splinter (voice) |
 Ayo EdebiriApril O'Neil (voice) | April O'Neil (voice) |