 Micah AbbeyDonatello (voice) | Donatello (voice) |
 Shamon Brown Jr.Michelangelo (voice) | Michelangelo (voice) |
 Nicolas CantuLeonardo (voice) | Leonardo (voice) |
 Brady NoonRaphael (voice) | Raphael (voice) |
 Ayo EdebiriApril O'Neil (voice) | April O'Neil (voice) |
 Maya RudolphCynthia Utrom (voice) | Cynthia Utrom (voice) |
 John CenaRocksteady (voice) | Rocksteady (voice) |
 Seth RogenBebop (voice) | Bebop (voice) |
 Rose ByrneLeatherhead (voice) | Leatherhead (voice) |
 Natasia DemetriouWingnut (voice) | Wingnut (voice) |
 Giancarlo EspositoBaxter Stockman (voice) | Baxter Stockman (voice) |
 Jackie ChanSplinter (voice) | Splinter (voice) |
 Ice CubeSuperfly (voice) | Superfly (voice) |
 Paul RuddMondo Gecko (voice) | Mondo Gecko (voice) |
 Post MaloneRay Fillet (voice) | Ray Fillet (voice) |
 Hannibal BuressGenghis Frog (voice) | Genghis Frog (voice) |
 Jimmy DonaldsonTimes Square Guy (voice) | Times Square Guy (voice) |
 Derek WilsonSpider (voice) | Spider (voice) |
| LLukas WilliamsCurious Goon (voice) | Curious Goon (voice) |
 Michael BadaluccoBad Bernie (voice) | Bad Bernie (voice) |
 Dempsey PappionBald Bronson (voice) | Bald Bronson (voice) |
 John CapodiceCab Driver (voice) | Cab Driver (voice) |
 Andia WinslowTV Anchor (voice) | TV Anchor (voice) |
 Raechel WongNews Anchor (voice) | News Anchor (voice) |
 David FaustinoNormal Nate (voice) | Normal Nate (voice) |
 Danny MastrogiorgioToupee Tom / Police Officer (voice) | Toupee Tom / Police Officer (voice) |
| NNoel GibsonResponding Police Officer / Military Advisor (voice) | Responding Police Officer / Military Advisor (voice) |
| MMyra OwyangShort Sharon (voice) | Short Sharon (voice) |
 Kevin EastmanGood Human (voice) | Good Human (voice) |
 Alex HirschBossy Goon / Scumbug (voice) | Bossy Goon / Scumbug (voice) |
