The four turtles travel back in time to the days of the legendary and deadly samurai in ancient Japan, where they train to perfect the art of becoming one. The turtles also assist a small village in an uprising.
| Actor | Role |
|---|---|
 Brian TochiLeonardo (voice) | Leonardo (voice) |
 Tim KelleherRaphael (voice) | Raphael (voice) |
 Corey FeldmanDonatello (voice) | Donatello (voice) |
 Robbie RistMichelangelo (voice) | Michelangelo (voice) |
 Paige TurcoApril O'Neil | April O'Neil |
 Elias KoteasCasey Jones / Whit | Casey Jones / Whit |
 James MurraySplinter | Splinter |
 Sab ShimonoLord Norinaga | Lord Norinaga |
 Stuart WilsonWalker | Walker |
 Vivian WuMitsu | Mitsu |
 Eidan HanzeiKenshin | Kenshin |
 John AylwardNiles | Niles |
 Mark CasoLeonardo | Leonardo |
 Matt HillRaphael | Raphael |
| JJim RaposaDonatello | Donatello |
 David FraserMichaelangelo | Michaelangelo |
 Mak TakanoBenkei - Honor Guard #1 | Benkei - Honor Guard #1 |
 Steve AkahoshiHonor Guard #2 | Honor Guard #2 |
| TTravis A. MoonYoshi | Yoshi |
| KKent KimHonor Guard #3 | Honor Guard #3 |
 Ken KenseiHonor Guard #4 | Honor Guard #4 |
 Tad HorinoGrandfather | Grandfather |
 Glen ChinJailer | Jailer |
 Koichi SakamotoYoung Priest | Young Priest |
| TTracy ConklinSam | Sam |
| EEdmund StoneDave | Dave |
| JJeff KawasugiMurota | Murota |
| PPhil ChongRider | Rider |
| YYeon KimBlacksmith | Blacksmith |