When Doc Ock and Lex Luthor join to defeat their nemesis, Spidey and the Man of Steel meet for the first time and must work together to foil the evil duo's nefarious plans for world domination.
| Actor | Role |
|---|---|
 Xander CorvusSpider-Man / Peter Parker | Spider-Man / Peter Parker |
 Ryan DrillerSuperman / Clark Kent | Superman / Clark Kent |
 Andy San DimasLois Lane | Lois Lane |
 Alexis TexasEve Teschmacher | Eve Teschmacher |
 Kagney Linn KarterSupergirl | Supergirl |
 Jenna PresleySpider-Woman | Spider-Woman |
 Capri AndersonMary Jane Watson | Mary Jane Watson |
 Lily LaBeauLiz Osborn | Liz Osborn |
 Jazy BerlinBlack Cat | Black Cat |
 Sarah ShevonBetty Brant | Betty Brant |
 Eric MastersonLex Luther | Lex Luther |
 James BartholetDoctor Octopus | Doctor Octopus |
 Michael VegasHarry Osborn | Harry Osborn |
 William BibbianiWilly | Willy |
 Rob BlackJ. Jonah Jameson (as Rob Black) | J. Jonah Jameson (as Rob Black) |
| WWill RyderPerry White | Perry White |