 William ShatnerAdmiral James T. Kirk | Admiral James T. Kirk |
 Leonard NimoyCaptain Spock | Captain Spock |
 Ricardo MontalbanKhan Noonien Singh | Khan Noonien Singh |
 DeForest KelleyDr. Leonard 'Bones' McCoy | Dr. Leonard 'Bones' McCoy |
 James DoohanCmdr. Montgomery 'Scotty' Scott | Cmdr. Montgomery 'Scotty' Scott |
 Walter KoenigCmdr. Pavel Chekov | Cmdr. Pavel Chekov |
 George TakeiCmdr. Hikaru Sulu | Cmdr. Hikaru Sulu |
 Nichelle NicholsCmdr. Uhura | Cmdr. Uhura |
 Bibi BeschDr. Carol Marcus | Dr. Carol Marcus |
 Merritt ButrickDr. David Marcus | Dr. David Marcus |
 Paul WinfieldCaptain Clark Terrell | Captain Clark Terrell |
 Kirstie AlleyLt. Saavik | Lt. Saavik |
 Judson ScottJoachim | Joachim |
 Ike EisenmannPreston | Preston |
 John VargasJedda | Jedda |
 John WinstonKyle | Kyle |
 Paul KentBeach | Beach |
 Nicholas GuestCadet | Cadet |
| RRussell TakakiMadison | Madison |
 Kevin Rodney SullivanMarch | March |
 Joel MarstonCrew Chief | Crew Chief |
| LLaura BanksKhan's Navigator | Khan's Navigator |
 Steve BondKhan's Crewman #1 | Khan's Crewman #1 |
 Brett Baxter ClarkKhan's Crewman #2 | Khan's Crewman #2 |
 Gilbert B. CombsEnterprise Engineer | Enterprise Engineer |
 James HornerEnterprise Crewman | Enterprise Crewman |
| GGeorge SasakiCrewmember at Spock's Funeral (uncredited) | Crewmember at Spock's Funeral (uncredited) |
| TTeresa E. VictorBridge Voice | Bridge Voice |
| DDianne HarperRadio Voice | Radio Voice |
 David RuprechtRadio Voice (credited as David Ruprecht) | Radio Voice (credited as David Ruprecht) |
