 Christopher Daniel BarnesPeter Parker / Spider-Man (voice) | Peter Parker / Spider-Man (voice) |
 Jennifer HaleFelicia Hardy / Black Cat (voice) | Felicia Hardy / Black Cat (voice) |
 Rodney SaulsberryJoe 'Robbie' Robertson / Security Guard / Paramedic (voice) | Joe 'Robbie' Robertson / Security Guard / Paramedic (voice) |
 Ed AsnerJ Jonah Jameson (voice) | J Jonah Jameson (voice) |
 Efrem Zimbalist Jr.Otto Octavius / Doctor Octopus / Butler (voice) | Otto Octavius / Doctor Octopus / Butler (voice) |
 Rue McClanahanMrs. Hardy (voice) | Mrs. Hardy (voice) |
| SSteven PolinskySecurity Chief / FBI Agent (voice) | Security Chief / FBI Agent (voice) |
 David HayterBrace Huntington (voice) | Brace Huntington (voice) |
 John Phillip LawJohn Hardesky / The Cat (voice) | John Hardesky / The Cat (voice) |
 Nita TalbotAnastasia Hardy (voice) | Anastasia Hardy (voice) |
 Roscoe Lee BrowneKingpin (voice) | Kingpin (voice) |
 David WarnerLandon (voice) | Landon (voice) |
 Jack AngelNick Fury (voice) | Nick Fury (voice) |
 Rachel DaviesAgent X (voice) | Agent X (voice) |
 Gary ImhoffHarry Osborn (voice) | Harry Osborn (voice) |
 Maxwell CaulfieldAlistair Smythe (voice) | Alistair Smythe (voice) |
| CCannon YoungChild Silvermane (voice) | Child Silvermane (voice) |
 Leigh-Allyn BakerAlisha Silvermane (voice) | Alisha Silvermane (voice) |
 Eddie AlbertAdam Toomes / Old Vulture (voice) | Adam Toomes / Old Vulture (voice) |
 Richard MollScorpion (voice) | Scorpion (voice) |
 Alan JohnsonArthur Avis / Young Vulture (voice) | Arthur Avis / Young Vulture (voice) |
 Sandra BernhardSarah Baker (voice) | Sarah Baker (voice) |
 Joseph CampanellaDoctor Curt Connors (voice) | Doctor Curt Connors (voice) |
 Townsend ColemanMiddle-Aged Silvermane (voice) | Middle-Aged Silvermane (voice) |
 Jeff CoreySilverman (voice) | Silverman (voice) |
