 Christopher Daniel BarnesPeter Parker / Spider-Man (voice) | Peter Parker / Spider-Man (voice) |
 Hank AzariaEddie Brock / Venom (voice) | Eddie Brock / Venom (voice) |
 Sara BallantineMary Jane Watson (voice) | Mary Jane Watson (voice) |
 Ed AsnerJ. Jonah Jameson (voice) | J. Jonah Jameson (voice) |
 James AveryJim Rhodes / War Machine (voice) | Jim Rhodes / War Machine (voice) |
 Roscoe Lee BrowneKingpin (voice) | Kingpin (voice) |
 Scott CleverdonCletus Kasady / Carnage (voice) | Cletus Kasady / Carnage (voice) |
 Robert HaysTony Stark / Iron Man (voice) | Tony Stark / Iron Man (voice) |
 Joseph CampanellaDr. Curt Connors (voice) | Dr. Curt Connors (voice) |
 Linda GaryAunt May (voice) | Aunt May (voice) |
 Jennifer HaleFelicia Hardy (voice) | Felicia Hardy (voice) |
 Rodney SaulsberryJoe 'Robbie' Robertson (voice) | Joe 'Robbie' Robertson (voice) |
 Patrick LabyorteauxFlash Thompson (voice) | Flash Thompson (voice) |
 Maxwell CaulfieldAlistair Smythe (voice) | Alistair Smythe (voice) |
 Don StarkRhino (voice) | Rhino (voice) |
 Michael HortonJohn Jameson (voice) | John Jameson (voice) |
| JJohn BeardNewscaster (voice) | Newscaster (voice) |
 Jim CummingsShocker (voice) | Shocker (voice) |
 Majel BarrettAnna Watson (voice) | Anna Watson (voice) |
 Tony JayBaron Mordo (voice) | Baron Mordo (voice) |
 Barbara GoodsonDr. Ashley Kafka (voice) | Dr. Ashley Kafka (voice) |
 Ed GilbertDormammu (voice) | Dormammu (voice) |
 Dawnn LewisDetective Terri Lee (voice) | Detective Terri Lee (voice) |
 Joan LeeMadame Web (voice) | Madame Web (voice) |
| LLiz GeorgesDebra Whitman (voice) | Debra Whitman (voice) |
