 Christopher Daniel BarnesPeter Parker / Spider-Man (voice) | Peter Parker / Spider-Man (voice) |
 Sara BallantineMary Jane Watson (voice) | Mary Jane Watson (voice) |
 Efrem Zimbalist Jr.Doctor Octopus (voice) | Doctor Octopus (voice) |
 Neil RossNorman Osborn / Green Goblin (voice) | Norman Osborn / Green Goblin (voice) |
| BBilly 'Pop' AtmoreRobert Farrell / Rocket Racer (voice) | Robert Farrell / Rocket Racer (voice) |
 Ed AsnerJ. Jonah Jameson (voice) | J. Jonah Jameson (voice) |
 Gary ImhoffHarry Osborn (voice) | Harry Osborn (voice) |
 Joan LeeMadame Web (voice) | Madame Web (voice) |
 Dimitra ArlissAnastasia Hardy (voice) | Anastasia Hardy (voice) |
 Linda GaryAunt May (voice) | Aunt May (voice) |
 Jennifer HaleFelicia Hardy (voice) | Felicia Hardy (voice) |
 Michael Des BarresJackson Weele (voice) | Jackson Weele (voice) |
 Majel BarrettAnna Watson (voice) | Anna Watson (voice) |
 Rodney SaulsberryJoe 'Robbie' Robertson (voice) | Joe 'Robbie' Robertson (voice) |
 Marla RubinoffLiz Allen (voice) | Liz Allen (voice) |
 Patrick LabyorteauxFlash Thompson (voice) | Flash Thompson (voice) |
| SStephanie EustaseMaria Taina Elizando (voice) | Maria Taina Elizando (voice) |
 Michael RyeDoctor Farley Stillwell (voice) | Doctor Farley Stillwell (voice) |
 Roscoe Lee BrowneWilson Fisk / Kingpin (voice) | Wilson Fisk / Kingpin (voice) |
 Anne-Marie JohnsonMousie (voice) | Mousie (voice) |
 Scott CleverdonDoctor Michael Pingree (voice) | Doctor Michael Pingree (voice) |
 Philip AbbottWardell Stromm (voice) | Wardell Stromm (voice) |
 Dawnn LewisDetective Terri Lee (voice) | Detective Terri Lee (voice) |
 Telma HopkinsMrs. Farrell (voice) | Mrs. Farrell (voice) |
 Mark HamillJason Phillips (voice) | Jason Phillips (voice) |