A young man with spider like abilities fights crime as a superhero in New York City while trying to have a normal personal life.
| Actor | Role |
|---|---|
 Neil Patrick HarrisPeter Parker/Spider-Man(voice) | Peter Parker/Spider-Man(voice) |
 Lisa LoebMary Jane Watson(voice) | Mary Jane Watson(voice) |
 Ian ZieringHarry Osborn(voice) | Harry Osborn(voice) |
 Angelle BrooksIndira 'Indy' Daimonji(voice) | Indira 'Indy' Daimonji(voice) |