 Christopher Daniel BarnesPeter Parker / Spider-Man (voice) | Peter Parker / Spider-Man (voice) |
 Sara BallantineMary Jane Watson (voice) | Mary Jane Watson (voice) |
 Ed AsnerJ. Jonah Jameson (voice) | J. Jonah Jameson (voice) |
 Roscoe Lee BrowneWilson Fisk / Kingpin (voice) | Wilson Fisk / Kingpin (voice) |
 Jennifer HaleFelicia Hardy (voice) | Felicia Hardy (voice) |
 Linda GaryAunt May (voice) | Aunt May (voice) |
 Julie BennettAunt May (voice) | Aunt May (voice) |
 Wanda De JesusDoctor Sylvia Lopez (voice) | Doctor Sylvia Lopez (voice) |
 Mark HamillJason Phillips / Hobgoblin (voice) | Jason Phillips / Hobgoblin (voice) |
 Dorian HarewoodTombstone (voice) | Tombstone (voice) |
 Gary ImhoffHarry Osborn (voice) | Harry Osborn (voice) |
 Nick JamesonRichard Fisk (voice) | Richard Fisk (voice) |
| OOliver MuirheadDoctor Jonathan Ohn / The Spot (voice) | Doctor Jonathan Ohn / The Spot (voice) |
 Neil RossNorman Osborn / Green Goblin (voice) | Norman Osborn / Green Goblin (voice) |
 Rodney SaulsberryJoe 'Robbie' Robertson (voice) | Joe 'Robbie' Robertson (voice) |
 David WarnerLandon (voice) | Landon (voice) |
 Carl BallantineLenny Luntz (voice) | Lenny Luntz (voice) |
 Majel BarrettAnna Watson (voice) | Anna Watson (voice) |
 Bob BergenNed Leeds (voice) | Ned Leeds (voice) |
 Joseph CampanellaDoctor Curt Connors (voice) | Doctor Curt Connors (voice) |
 Nell CarterGlory Grant (voice) | Glory Grant (voice) |
| FFrankie CrockerJimmie Dugan (voice) | Jimmie Dugan (voice) |
| LLiz GeorgesDebra Whitman (voice) | Debra Whitman (voice) |
 Robert HaysStark (voice) | Stark (voice) |
 Patrick LabyorteauxFlash Thompson (voice) | Flash Thompson (voice) |
 Joan LeeMadame Web (voice) | Madame Web (voice) |
 Iona MorrisMartha Robertson (voice) | Martha Robertson (voice) |
 Lois NettletonNora (voice) | Nora (voice) |
 Alfonso RibeiroRandy Robertson (voice) | Randy Robertson (voice) |
| SStanley Ralph RossJoey Nails (voice) | Joey Nails (voice) |
